Triticum monococcum bacterial artificial chromosome (BAC) library
D. Lijavetzky, G. Muzzi, T. Wicker, B.
Keller, R. Wing, and J.
Dubcovsky.
Conditions and addresses to request clones and filters
Material transfer agreement to request clones and filters
· Plant material: cultivated diploid wheat Triticum monococcum DV92.
· High molecular weight DNA: nuclei extracted from leaves.
· Partial digestion: Hind III followed by double size-selection (Fig1 and 2).
· Cloning vector: pINDIGO451 BAC
· Library characteristics:
· 276,480 BAC clones sorted in 720 384-well plates and blotted onto 15 high-density filters
· Average insert size of 115 kb
· Empty clones: 1.33 %. Hybridization with labeled total DV92 DNA showed that all the other BAC clones have wheat DNA. 85% of the Not I fragments showed strong hybridization signal suggesting the presence of repetitive sequences.
· Clones with chloroplast DNA: 0.14 %
· Library coverage: 5.6 genome equivalents.
· Probability of having any DNA sequence represented in this library: > 99.6 %.