Triticum turgidum var. durum Bacterial artificial chromosome (BAC) library

A. Cenci^1,2, N. Chantret^1,3, K. Xy⁴, Y. Gu⁴, O.D. Anderson⁴, T. Fahima^1,5, A. Distelfeld^1,5and J. Dubcovsky.¹

^1. Dept. Agronomy and Range Science, Wheat Genomic Center, University of California, Davis, Ca, USA.
^2. Current address: Dip. Biologia e Chimica Agroforestale e Ambientale, Universita' degli Studi di Bari, Italia.
^3. Current address: Institute National de la Recherche Agronomique, Rennes, France.
^4. USDA Western Reg. Research Center, Wheat Genomic Center,  Albany, Ca, USA.
⁵ Current addresse: The Institute of Evolution, University of Haifa, Mt. Carmel, Haifa. Israel

Publication: Cenci, A., N. Chantret, K. Xy, Y. Gu, O.D. Anderson, T. Fahima, A. Distelfeld, and J. Dubcovsky. 2003. A half million clones Bacterial Artificial Chromosome (BAC) library of durum wheat. Theor. Appl. Genet. 107:931-939.

Funding: This project was supported by USDA-BARD grant US-3224-01R, by INRA-France and by USDA-Albany.

· Conditions and addresses to request clones and filters

· Material Transfer Agreement: To request clones or filters please print, sign and send to

J. Dubcovsky,

Agronomy & Range Science, University of California

One Shields Avenue, Davis, 95616 CA, USA

· Plant material: cultivated tetraploid wheat Triticum turgidum var. durum, cultivar Langdon with a 30 cM homozygous insertion of T. turgidum var. dicoccoides accession FA15-3 from chromosome 6BS carrying a gene for high grain protein content.

· High molecular weight DNA: nuclei extracted from leaves.

· Cloning vector: pIndigoBac536

· Library characteristics:

· 516,096 BAC clones picked in 1,344 384-well plates. Average insert size 130 kb

· High density filters production: 28 sets of high-density filters completed

· Empty clones <1%

· Chloroplast clones 0.34%

· Present library coverage (based on estimated DNA content of 13,000 Mbp/1C): 5X