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Here are imputed expression of 74464 genes based on similarity analysis between single-cell RNA-seq data and 264 variable genes obtained from 10x GENOMICS Xenium Platform for wheat apex at 2 stages (double ridge (W2.0) and floret primordia (W3.5) stages; each stage has two images) based on the methods decribed in Demesa-Arevalo et al. 2025.

All the gene IDs are from Kronos annotation v1.0. You can convert gene IDs between Chinese Spring RefSeq v1.1 annotation and Kronos v1.0 annotation here: https://junli.netlify.app/apps/cs-kronos-gene-match/. You can find the best hit of Kronos v1.0 annotation on Arabidopsis, rice, and barley here: https://junli.netlify.app/apps/wheat-homeolog/

Below is just a quick guide:

1. On the left panel (Cluster Map), you can view all or a few clusters.
2. On the right panel (Expression Heatmap), you can check single gene or a pair of gene expression in each cell.
3. "Gene 1" has heatmap for the cell fill color; "Gene 2" has heatmap for the cell border color. Values are raw counts per cell.
4. Select "Gene 1" or "Gene 2" will clear the cluster colors; and "Gene 1" competes the fill colors with the clusters.
5. Gene 2 and the clusters can be shown at the same time, but make sure to select "Gene 2" first, then select the clusters.
6. You can use the mouse wheel to zoom in or out; drag it to move.
7. You can also select cells in a region and get all its transcripts by clicking "Enable Cell Selection" button.
8. You can download a SVG (Scalable Vector Graphics) format image or a high-quality PNG image.
9. You can now check a list of genes quickly by pasating them in to the textbox on the bottom left corner; then go to top right corners and use the "Last Gene" and "Next Gene" button to check.
10. You can download Kronos and Chinese spring gene IDs of the 316 genes.