Wheat Target Induced Local Lesions In Genome (TILLING) project based on the tetraploid wheat line Triticum turgidum cv. Kronos. This project entails targeted re-sequencing of the protein coding regions of over 1500 EMS mutagenized individuals from the Kronos cultivar. In each wheat line, we have identified mutations in protein-coding regions and predicted their effect on protein function. All data is incorporated into a public database.
The Kronos genomic sequence is available to all researchers under the Toronto license, information in the release terms and here http://www.nature.com/nature/journal/v461/n7261/full/461168a.html
Genome assembly is available from here:
https://opendata.earlham.ac.uk/opendata/data/Triticum_turgidum/EI/v1.1
Account Registration (needed for seed order form access)
Order Form (REQUEST SEEDS)
Sample TILLING Data for Ensembl (new assembly)
Sample Search for OLD TILLING Data
Frequently Asked Questions - FAQs
Capture Design Reference FASTA
UCW Contigs from unmapped reads FASTA
TGAC Contigs for unmapped reads FASTA
GeneAnnotationSummary for Kronos
GeneAnnotationSummary for Cadenza
Krasileva K V, Vasquez-Gross HA, Howell T, Bailey P, Paraiso F, Clissold L, et al. Uncovering hidden variation in polyploid wheat. Proc. Natl. Acad. Sci. U. S. A. National Academy of Sciences; 2017 Available from: http://www.ncbi.nlm.nih.gov/pubmed/28096351