Wheat TILLING

Wheat Target Induced Local Lesions In Genome (TILLING) project based on the tetraploid wheat line Triticum turgidum cv. Kronos.  This project entails  targeted re-sequencing of the protein coding regions of over 1500 EMS mutagenized individuals from the Kronos cultivar. In each wheat line, we have identified mutations in protein-coding regions and predicted their effect on protein function. All data is incorporated into a public database.  

The Kronos genomic  sequence is available to all researchers under the Toronto license, information in the release terms and here http://www.nature.com/nature/journal/v461/n7261/full/461168a.html
 
Genome assembly is available from here:
https://opendata.earlham.ac.uk/opendata/data/Triticum_turgidum/EI/v1.1

Account Registration (needed for seed order form access)

Order Form (REQUEST SEEDS)

Documentation

Definitions

Wheat BLAST

Sample Search for TILLING Data

TILLING MTA Form

Frequently Asked Questions - FAQs

Financial Support

Capture Design Reference FASTA

UCW Contigs from unmapped reads FASTA

TGAC Contigs for unmapped reads FASTA

GeneAnnotationSummary for Kronos 

GeneAnnotationSummary for Cadenza

Krasileva K V, Vasquez-Gross HA, Howell T, Bailey P, Paraiso F, Clissold L, et al. Uncovering hidden variation in polyploid wheat. Proc. Natl. Acad. Sci. U. S. A. National Academy of Sciences; 2017 Available from: http://www.ncbi.nlm.nih.gov/pubmed/28096351